〔Abstract〕 Objective To explore the effect of bird's nest nursing position combined with auditory intervention on relieving the pain of airway suction during mechanical ventilation of newborns. Methods A total of 98 neonates with airway suction during mechanical ventilation treated in Zhengzhou University Affiliated Children's Hospital from March 2020 to October 2022 were selected and divided into an observation group and a control group according to random number table method, with 49 cases in each group. The control group was given routine analgesia, and the observation group was given bird's nest nursing position combined with auditory intervention. Neonatal heart rate, blood oxygen saturation, pain and parental satisfaction were compared between the two groups. Results After 3 days of mechanical ventilation intervention, the heart rate and blood oxygen saturation of neonates in the two groups were higher than those in mechanical ventilation. However, the heart rate of the observation group was lower than that of the control group, and the blood oxygen saturation was higher than that of the control group, with statistically significant difference (P < 0.05). After 3 days of mechanical ventilation intervention, premature infant pain profile scores of newborns in the two groups were lower than those in mechanical ventilation, and the observation group was lower than that of the control group, with statistically significant difference (P < 0.05). The total satisfaction of newborn parents in the observation group was higher than that in the control group, with statistically significant difference (P < 0.05). Conclusion The bird's nest nursing position combined with auditory intervention has an obvious effect on relieving the pain of airway suction during mechanical ventilation of newborns, and can significantly improve the level of newborn heart rate and blood oxygen saturation, and the satisfaction of newborn families is high.