电针膝三针对膝骨关节炎大鼠 TLR4 及 NF–κBp65 磷酸化水平变化的影响
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王珍珍,女,主治医师,主要研究方向是针灸。

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R 743.3

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广东省中医药局科研项目(20202086)


Effect of Electroacupuncture Knee Tri-acupuncture on the Expression of TLR4 and p-NF-κBp65 Protein in Rats with Knee Osteoarthritis
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    摘要:

    摘 要目的:探讨电针膝三针对膝骨关节炎大鼠 Toll 样受体 4(TLR4)及核因子 κBp65(NF–κBp65)磷酸化水平变 化的影响。 方法:选取 40 只 8 月龄大鼠,10 只为对照组,其余 30 只采用后肢跟腱切除法造模。造模后平均分为模型组、 电针组和塞来昔布组,8 周后取材。肉眼和光镜下观察各组大鼠膝软骨组织形态学变化,分别通过 Moran 评分、Mankin 评 分判定软骨病变程度;用 Western blot 法分别检测各组大鼠软骨 TLR4、NF–κBp65 磷酸化蛋白(p–NF–κBp65)以及白细胞 介素 –6(IL–6)、肿瘤坏死因子 –α(TNF–α)含量变化。 结果:模型组 Moran 评分低于对照组,Mankin 评分高于对照组; 而电针组和塞来昔布组 Moran 评分高于模型组,Mankin 评分低于模型组。模型组大鼠 TLR4、p–NF–κBp65、IL–6、TNF–α 表达水平高于对照组,而电针组和塞来昔布组 TLR4、p–NF–κBp65、IL–6、TNF–α 表达水平低于模型组。 结论:电针膝三 针可减少膝关节炎大鼠软骨破坏,延缓软骨退变,其作用机制可能是通过下调 TLR4/NF–κBp65 通路关键调节因子 TLR4、 p-NF–κBp65 的表达,减少炎症因子 IL–6、TNF–α 的释放,从而延缓膝骨关节炎的发展进程。

    Abstract:

    AbstractObjective To investigate the effect of electroacupuncture (EA) Knee Tri-acupuncture on knee osteoarthritis(KOA) rats and the expression of Toll-like receptor 4,(TLR4) and nuclear factor κBp65 (NF-κBp65). Methods A total of 76 patients with CHD and tachyarrhythmia diagnosed and treated in Pingdingshan Hospital of Traditional Chinese Medicine from July 2020 to December 2022 were selected and divided into a control group and an observation group according to random number table method, with 38 cases in each group. The control group was treated with metoprolol according to the conventional treatment plan, and the observation group was treated with Yangxin Yiqi Tongmai decoction combined with metoprolol. The clinical efficacy, traditional chinese medicine syndrome score, hemorheology index and occurrence of adverse reactions were compared between the two groups. Results Moran score in the model group was significantly lower than that in the control group, while Mankin score was significantly higher than that in the control group . Moran scores in electroacupuncture group and celecoxib group were significantly higher than those in model group, while Mankin score were significantly lower than those in the control group. The expression levels of TLR4, p-NF-κBp65, IL-6 and TNF-α in the model group were significantly higher than those in the control group, while the expression levels of TLR4, p-NF-κBp65, IL-6 and TNF-α in the electroacupuncture and celecxib groups were significantly lower than those in the model group. Conclusion EA Knee Tri-acupuncture can reduce the cartilage destruction and delay the degeneration of articular cartilage in the rat model of KOA, which may be related to its effects in down-regulating the levels of TLR4 and p-NF-κBp65 and reduce the release of inflammatory factors IL-6 and TNF-α.

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  • 收稿日期:2023-12-09
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  • 在线发布日期: 2024-06-28
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