柴胡疏肝散含药血清对衣霉素诱导神经元 内质网应激模型细胞凋亡的影响
作者:
作者单位:

作者简介:

李芷悦,女,医学博士,中国科学院深圳先进技术研究院博士后,主要从事中医药防治老年慢病作用 机理研究。

通讯作者:

中图分类号:

R 285.5

基金项目:

国家自然科学基金资助项目(81574038);中国博士后基金项目(2023M732373)


Effect of Chaihu Shugan San Containing Serum on Apoptosis in a Model of Neuronal Endoplasmic Reticulum Stress induced by Tetracycline
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    摘 要目的:探讨柴胡疏肝散(CSS)含药血清对衣霉素诱导的内质网应激(ERS)模型神经元凋亡的影响。 方法:采用衣霉素对 NG108–15 神经细胞进行诱导,建立 ERS 细胞模型并分成 5 组,模型组(10 % 空白兔血清 干预)、安理申(盐酸多奈哌齐片)组(10 % 安理申含药血清干预)、2.5 % CSS 含药血清(低剂量)组、5 % CSS 含药血清(中剂量)组、10 % CSS 含药血清(高剂量)组。同时设置正常空白血清孵育且未经衣霉素诱导的正常组, 干预后采用流式细胞仪和荧光染色检测各组细胞凋亡率。 结果:流式细胞仪检测结果显示,各干预组细胞凋亡率均低 于模型组(P < 0.05);同时高剂量 CSS 含药血清组细胞凋亡率明显低于中低剂量组(P < 0.05)。联合荧光染色 也证明高剂量 CSS 含药血清组细胞凋亡率明显低于中低剂量组。 结论:CSS 能够抑制衣霉素诱导的 ERS 模型神经元 凋亡,且其抑制作用随着药物浓度升高而增强。

    Abstract:

    AbstractObjective To explore the effect of Chaihu Shugan San (CSS) containing serum on neuronal apoptosis in induced by tunicamycin of endoplasmic reticulum stress (ERS) model. Methods The NG108-15 neural cells were induced with tetracycline in the experiment, and an ERS cell model was established and divided into 5 groups: model group (10% blank rabbit serum intervention), donepezil hydrochloride table group (10% donepezil hydrochloride table drug serum intervention), 2.5% CSS drug serum (low-dose) group, 5% CSS drug serum (medium dose) group, and 10% CSS drug serum (high-dose) group. At the same time, a normal group was incubated with normal blank serum and not induced by tetracycline. After completing the observations, flow cytometry and fluorescence staining were performed to detect the apoptosis rate of cells in each group. Results The flow cytometry detection results showed that the apoptosis rate of each intervention group was significantly reduced compared to the model group (P < 0.05). At the same time, the apoptosis rate of cells in the high-dose CSS serum containing group was significantly lower than that in the medium-dose and low-dose group (P < 0.05). Combined fluorescence staining also demonstrated that the apoptosis rate of cells in the high-dose CSS serum group was significantly lower than that in the medium-close and low-dose group. Conclusion CSS has a significant protective effect on neuronal damage caused by ERS, and can inhibit neuronal apoptosis in ERS. Its inhibitory effect increases with increasing drug concentration.

    参考文献
    相似文献
    引证文献
引用本文
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2023-12-15
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2024-06-28
  • 出版日期:
重要通知

近期有不法分子冒充我刊名义给作者打电话或发邮件,编造各种理由要求添加微信或QQ、伪造复制我刊编辑部公章发放假冒录用通知书等等各种方式试图骗取作者钱财。我刊在此郑重声明:(1)杂志社与作者沟通方式为电话和邮件,在杂志社官方网站(http://www.szzxyjh.com)“联系我们”下拉菜单,或者网站最下端信息栏可以查询),绝对不会要求作者添加微信或QQ。此网站为我刊唯一官方网站,其他均为假冒网站。(2)我刊录用稿件仅收取合理版面制作费和审稿费,收款单位为“深圳市第二人民医院”对公账户,其他涉及私人账户者均为假冒。望广大作者提高警惕,谨防上当。谢谢配合!
《深圳中西医结合杂志》编辑部

关闭