〔摘 要〕 目的：建立天麻安神汤主要药味的薄层鉴别和君药天麻中天麻素和对羟基苯甲醇的同时测定方法，用于对该 方剂进行准确质量控制和评价。 方法：对天麻和首乌藤使用薄层色谱法（TLC）鉴别，含量测定采用高效液相色谱法（HPLC）， Agilent TC C18 色谱柱为固定相，以乙腈 –0.05 % 磷酸溶液（3:97）为流动相，流速 1.0 mL·min-1，检测波长 220 nm， 柱温 25 ℃，进样量 10 μL，以天麻素和对羟基苯甲醇平行制备 10 批天麻安神汤进行测定。 结果：10 批样品进行含量测定 结果显示，天麻素的含量为 0.0311 ~ 0.0365 mg·mL-1，对羟基苯甲醇为 0.0210 ~ 0.0245 mg·mL-1 。 结论：对天麻安神汤君 药天麻中天麻素和对羟基苯甲醇进行了同时测定，方法快速、准确、高效，为该方剂的质量精准控制提供了科学依据。
〔Abstract〕 Objective The thin-layer identification of main medicinal tastes of Tianma Anshen Decoction and the simultaneous determination of gastrodin and p-hydroxybenzyl alcohol in Tianma Anshen Decoction were established for the accurate quality control and evaluation of the prescription. Methods The identification of Gastrodia and Nightfork was performed by thin layer chromatography (TLC), and the content was determined by high performance liquid chromatography (HPLC). Agilent TC C18 column was used as stationary phase, and acetonitrile-0.05% phosphoric acid solution (3:97) as the mobile phase, the flow rate was 1.0 mL·min-1, the detection wavelength was 220 nm, the column temperature was 25 ℃, and the sample size was 10 μL. 10 batches of Tianma Anshen decoction were prepared by parallel preparation of gastrodin and p-hydroxybenzyl alcohol. Results The results of 10 batches of samples showed that the content of gastrodin was 0.0311 ~ 0.0365 mg·mL-1 and p-hydroxybenzyl alcohol was 0.0210 ~ 0.0245 mg·mL-1. Conclusion The simultaneous determination of gastrodin and p-hydroxybenzyl alcohol in Gastrodia is carried out. The method is rapid, accurate and efficient, which provided scientific basis for the precise quality control of the prescription.