〔Abstract〕 Objective To screen long noncoding RNA (lncRNA) associated with MYCN expression in neuroblastoma (NB), analyzing its effect on the patients' prognosis, and finding potential therapeutic targets. Methods Gene expression data were downloaded from TARGET and GEO databases, and the two groups were divided into MYCN high expression group and low expression group, respectively. R language was used to screen the differentially expressed genes, and obtained common differentially expressed lncRNAs. The mRNAs interacting with those lncRNAs were predicted by public ENCORI database; DAVID database was used to analyze the functions of these genes. Survival analysis of the differentially expressed lncRNAs was performed by TARGET database. Results A total of 1104 the differentially expressed lncRNAs were screened out from TARGET database, 2849 differentially expressed genes were screened from geo database, 59 common differentially expressed lncRNAs were identified after intersection with GEO identified; and 547 mRNAs were predicted to interact with them by ENCORI database. These differentially expressed lncRNAs were significantly enriched in the protein binding, negative regulation of transcription from RNA polymerase II promoter, and Alzheimer disease signaling pathway. TARGET database was used for further survival analysis, showed that VPS9D1-AS1, LINC00649 and LINC01234 could affect the survival of patients. Conclusion Through bioinformatics analysis of differentially expressed lncRNAs related to MYCN expression, it was found that VPS9D1-AS1, LINC00649 and LINC01234 may be closely related to the occurrence and development of NB, providing direction and ideas for subsequent research.